Perforin Set anti-Human, FITC, Clone: +BD7238, BD

Description

• Perforin has a key role in cell-mediated cytotoxicity
• It is a 70 kDa cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells
• CTLs are involved in eliminating virally infected cells, in anti-tumor immune responses, in allograft rejections, and in some autoimmune diseases
• NK cells are important for tumor surveillance and destruction and are involved in allograft rejections
• Cytotoxic cells release the contents of their cytotoxic granules, including perforin upon recognition of their target cell
• In the presence of calcium, perforin forms transmembrane channels or pores in the membrane of the target cell leading to a cell death that resembles apoptosis
• The ability to detect perforin-positive cells with specific antibody should be useful in identifying and understanding perforin-mediated reactions
• Clone δ G9 reacts with human and bovine perforin
• It does not cross-react with mouse perforin
• Purified granules from the human lymphoma cell line YT were used as immunogen
• Clone δ G9 was initially characterized by immunoprecipitation and immunohistochemistry of frozen tissue sections
• The antibody stains scattered lymphocytes in red pulp of spleen, and scattered infiltrated lymphocytes in lymphoma.