Mouse B Lymphocyte Subset Antibody Cocktail, with Isotype Control; PE-Cy7 CD45R/B220, PE CD23 (FcηRII), & APC sIgM Mouse anti-Mouse, APC, PE, PE-Cy7, Clone: B3B4, RA3-6B2, II/41, BD

Description

• The Mouse B Lymphocyte Subset Antibody Cocktail is a three-color reagent designed to identify major subsets of B lymphocytes by direct immunofluorescent staining with flow cytometric analysis
• The RA3-6B2 antibody recognizes an epitope of the extracellular domain of CD45 that is primarily expressed, at developmentally regulated levels, on B lymphocytes at all stages from pro-B through mature, activated, antibody-secreting, and memory B cells
• Although CD45R/B220 has been considered to be a defining antigen of the B-cell lineage, lytically active NK cells, some activated or apoptotic T cells, and some non-B-lineage hematopoietic progenitors have been reported to express CD45R/B220
• The B3B4 antibody recognizes CD23, the low-affinity IgE Fc receptor that is expressed on mature resting conventional B cells, but not on B-1 cells (CD5+ B lymphocytes), T lymphocytes, or mast cells
• The II/41 antibody recognizes the surface IgM (sIgM), specifically immunoglobulin chain, which is a component of the antigen receptor complex on immature and mature B lymphocytes, including plasma cells
• The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance
• The Mouse B Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulin
• The use of three different fluorochromes for the labelling of the three different antibodies permits the recognition of each of the three antigens on each cell in a sample
• The levels of expression of the three antigens distinguish the major subpopulations of developing and peripheral B lymphocytes
• Additional fluorochrome-labelled reagents may be combined with the Mouse B Lymphocyte Subset Antibody Cocktail, and the Mouse B Lymphocyte Subset Isotype Control, to further characterize B-cell subpopulations.