BDB558332

Mouse B Lymphocyte Subset Antibody Cocktail, with Isotype Control; PE-Cy7 CD45R/B220, PE CD23 (FcηRII), & APC sIgM Mouse anti-Mouse, APC, PE, PE-Cy7, Clone: B3B4, RA3-6B2, II/41, BD

Manufacturer: BD Biosciences

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Pack Size SKU Availability Price
Each of 1 BDB558332-Each-of-1 In Stock ₹ 59,036.40

BDB558332 - Each of 1

₹ 59,036.40

In Stock

Quantity

1

Base Price: ₹ 59,036.40

GST (18%): ₹ 10,626.552

Total Price: ₹ 69,662.952

Antigen

CD45R/B220, PE CD23 (FcηRII), & APC sIgM

Classification

Monoclonal

Conjugate

APC/PE/PE-Cyanine7

Purification Method

Affinity Purified

Regulatory Status

RUO

Includes

Mouse B Lymphocyte Subset Antibody Cocktail; PE-Cy7 CD45R/B220, PE CD23, and APC sIgM (100 tests), Mouse B Lymphocyte Subset Isotype Control; PE-Cy7, PE, and APC Rat IgG2a, kappa (100 tests)

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications

Flow Cytometry

Clone

B3B4, RA3-6B2, II/41

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Mouse

Description

  • The Mouse B Lymphocyte Subset Antibody Cocktail is a three-color reagent designed to identify major subsets of B lymphocytes by direct immunofluorescent staining with flow cytometric analysis
  • The RA3-6B2 antibody recognizes an epitope of the extracellular domain of CD45 that is primarily expressed, at developmentally regulated levels, on B lymphocytes at all stages from pro-B through mature, activated, antibody-secreting, and memory B cells
  • Although CD45R/B220 has been considered to be a defining antigen of the B-cell lineage, lytically active NK cells, some activated or apoptotic T cells, and some non-B-lineage hematopoietic progenitors have been reported to express CD45R/B220
  • The B3B4 antibody recognizes CD23, the low-affinity IgE Fc receptor that is expressed on mature resting conventional B cells, but not on B-1 cells (CD5+ B lymphocytes), T lymphocytes, or mast cells
  • The II/41 antibody recognizes the surface IgM (sIgM), specifically immunoglobulin chain, which is a component of the antigen receptor complex on immature and mature B lymphocytes, including plasma cells
  • The three antibodies have been titrated and pre-diluted, mixed together, and formulated for optimal staining performance
  • The Mouse B Lymphocyte Subset Isotype Control contains equivalent concentrations of fluorochrome- and isotype-matched negative-control immunoglobulin
  • The use of three different fluorochromes for the labelling of the three different antibodies permits the recognition of each of the three antigens on each cell in a sample
  • The levels of expression of the three antigens distinguish the major subpopulations of developing and peripheral B lymphocytes
  • Additional fluorochrome-labelled reagents may be combined with the Mouse B Lymphocyte Subset Antibody Cocktail, and the Mouse B Lymphocyte Subset Isotype Control, to further characterize B-cell subpopulations.

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Antigen:
CD45R/B220, PE CD23 (FcηRII), & APC sIgM

Classification:
Monoclonal

Conjugate:
APC/PE/PE-Cyanine7

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Includes:
Mouse B Lymphocyte Subset Antibody Cocktail; PE-Cy7 CD45R/B220, PE CD23, and APC sIgM (100 tests), Mouse B Lymphocyte Subset Isotype Control; PE-Cy7, PE, and APC Rat IgG2a, kappa (100 tests)

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
B3B4, RA3-6B2, II/41

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Mouse

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Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Applications:
Flow Cytometry

Clone:
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Host Species:
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Quantity:
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Primary or Secondary:
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Content And Storage:
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Store undiluted at 4°C

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