EGF Receptor (pY1173) Mouse, Alexa Fluor 647, Clone: 9H2, BD

Description

• Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF Receptor (EGFR) binding and activation of intrinsic tyrosine kinase activity
• EGFR, also known as ErbB1 or HER1, is a member of the ErbB class of receptor protein tyrosine kinases
• It has an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic region containing a protein tyrosine kinase domain and a c-terminal regulatory domain with many phosphorylation sites
• Following ligand binding, EGFR forms homodimers and heterodimers with ErbB2
• Specific C-terminal tyrosine residues are then autophosphorylated and, in turn, bind to adaptor proteins, kinases, or protein tyrosine phosphatases
• Specifically, phosphorylated tyrosine 1173 (Y1173) interacts with Shc, SHP1, and PLCγ, which mediate downstream signaling cascades and negative feedback regulation of EGFR activation
• Inappropriate expression or mutations of EGFR and/or deregulation of its signaling pathways are associated with many types of cancer, making EGFR a promising target for cancer therapies
• The 9H2 monoclonal antibody recognizes the phosphorylated Y1173 in the regulatory domain of human EGFR
• Intracellular Staining