Btk Mouse anti-Human, Alexa Fluor 647, Clone: 53/BTK, BD

Description

• B ruton's t yrosine k inase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling
• It is a member of the Tec family of kinases which includes Tec and Itk
• This family is similar to the src family of tyrosine kinases
• However, Tec family members lack the N-terminal myristylation site and the regulatory C-terminal tyrosine that are found in src proteins
• In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain
• The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551
• This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain
• Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate
• The PH domain is essential for proper activation and function of Btk
• A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.