Stat1 (N-Terminus) Mouse, Alexa Fluor 647, Clone: 1/STAT1, BD

Description

• Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors
• Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation
• Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes
• Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner
• Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor
• Stat1 has two alternatively spliced isoforms, 91kDa Stat1α and 84kDa Stat1β Stat1α has 38 additional C-terminal amino acids
• In response to the binding of IFNα, IFNγ, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus
• This results in the formation of an active complex that includes the DNA-binding p48 subunit
• This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs)
• Thus, phosphorylation of Y701 in Stat1 occurs in response to growth factors and cytokines, and is essential for normal transcriptional activity of the ISGF3 complex
• The 1/Stat1 monoclonal antibody recognizes the N-terminus of human Stat1 (both isoforms), regardless of phosphorylation status
• Intracellular Staining