4EBP1 (pT36/pT45) Mouse, Alexa Fluor 488, Clone: M31-16, BD

Description

• The eukaryotic translation initiation factor 4E - B inding P rotein 1 (4EBP1) is a p hosphorylated h eat- and a cid- s table protein (PHAS-I or PHAS-1), and it is regulated by insulin
• It is a member of the eIF4E-Binding Protein Family, which also includes the proteins 4EBP2 and 4EBP3
• 4EBP1 binds with e ukaryotic translation I nitiation F actor 4E (eIF4E), which prevents its assembly into the eIF4E complex and inhibits cap-dependent translation
• When 4EBP1 is phosphorylated, this binding is disrupted, allowing cap-dependent translation to be activated
• Phosphorylation of 4EBP1 is required for protein synthesis, and it mediates the regulation of protein translation by stimuli that signal through the phosphoinositide 3 (PI3) kinase pathway
• We found that threonines 36 and 45 (T36/T45) are phosphorylated in resting human peripheral blood mononuclear cells
• PI3 kinase inhibitors, such as LY294002 down-regulate the phosphorylation level of 4EBP1 (pT36/pT45)
• The M31-16 monoclonal antibody recognizes the phosphorylated T36 and T45 of activated human 4EBP1
• The orthologous phosphorylation sites in mouse and rat 4EBP1 are T35 and T44.