SSEA-1 Mouse, Brilliant Violet 421, Clone: MC480, BD

Description

• The MC480 monoclonal antibody reacts with S tage- S pecific E mbryonic A ntigen-1 (SSEA-1), which is a terminal carbohydrate epitope (3-fucosyl-N-acetyllactosamine or 3-FAL) on glycoproteins and lactose series glycolipids
• SSEA-1 is related to Lewis blood group antigens and is found in a variety of embryonic and adult tissues and cancers
• As its name implies, the expression of SSEA-1 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation
• However, its expression pattern differs in the human and mouse
• In the human, SSEA-1 is not found on embryonic stem (ES) cells, embryonic inner cell mass (ICM), or teratocarcinoma (embryonal carcinoma or EC) cells
• As human EC and ES cells undergo differentiation, SSEA-1 expression is upregulated
• In the adult, the same epitope is expressed as CD15 on granulocytes and monocytes, but not lymphocytes or dendritic cells
• In the mouse, SSEA-1 is found on EC, ES, and primordial germ cells, 8-cell to blastocyst embryos, ICM, and on subpopulations of cells in the adult central nervous system, including stem cells
• In contrast to the human, SSEA-1 expression is reduced as mouse EC and ES cells undergo differentiation
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.