SSEA-4 Mouse, BUV395, Clone: MC813-70, BD

Description

• The MC813-70 monoclonal antibody reacts with S tage- S pecific E mbryonic A ntigen- 4 (SSEA-4), a carbohydrate epitope on the major ganglioside, but not the neutral glycolipid, of human teratocarcinoma cells
• As its name implies, the expression of SSEA-4 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation
• However, its expression pattern differs in the human and mouse
• In the human, SSEA-4 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (ICM), embryonic stem (ES) cells, and the K562 erythromyeloid leukeumia cell line
• As human stem cells undergo differentiation, SSEA-4 expression is lost
• In the mouse, SSEA-4 is found on oocytes and early cleavage-stage embryos, and primitive ectoderm, but not on EC, ICM, or ES cells
• In some cases, SSEA-4 expression appears upon differentiation of mouse EC or ES cells
• The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument
• With an Ex Max at 348 nmand an Em Max at 395nm, this dye has virtually no spillover into any other detector
• BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.