TRA-1-60 Mouse anti-Human, BUV395, Clone: TRA-1-60, BD

Description

• The TRA-1-60 monoclonal antibody reacts with the neuraminidase-resistant form of a pluripotent-stem-cell-specific epitope on a high-molecular-weight transmembrane glycoprotein
• The TRA-1-60 antigen is a sialylated epitope on the same keratan sulfate core molecule, podocalyxin, as 4 other distinct antigens on tumor-derived cell lines, TRA-1-81, GCTM2, K4, and K21
• The expression of TRA-1-60 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation
• The antigen is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (but not morula or trophoblast), and embryonic stem (ES) cells
• TRA-1-60 antigen is released into the serum of patients bearingtesticular tumors containing EC cells
• As human EC and ES cells undergo differentiation, expression of TRA-1-60 antigen is lost
• Expression of TRA-1-60 antigen has also been observed on a rhesus monkey ES cell line (Thomson et al, 1995)
• The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument
• With an Ex Max at 348 nmand an Em Max at 395nm, this dye has virtually no spillover into any other detector
• BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.