SSEA-4 Mouse, Brilliant Violet 605, Clone: MC813-70, BD

Description

• The MC813-70 monoclonal antibody reacts with S tage- S pecific E mbryonic A ntigen- 4 (SSEA-4), a carbohydrate epitope on the major ganglioside, but not the neutral glycolipid, of human teratocarcinoma cells
• As its name implies, the expression of SSEA-4 is stage-specific and can be used to characterize embryonic cells and monitor their differentiation
• However, its expression pattern differs in the human and mouse
• In the human, SSEA-4 is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (ICM), embryonic stem (ES) cells, and the K562 erythromyeloid leukeumia cell line
• As human stem cells undergo differentiation, SSEA-4 expression is lost
• In the mouse, SSEA-4 is found on oocytes and early cleavage-stage embryos, and primitive ectoderm, but not on EC, ICM, or ES cells
• In some cases, SSEA-4 expression appears upon differentiation of mouse EC or ES cells
• This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment
• Fluorescent dye interactions may cause staining artifacts which may affect data interpretation
• The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions
• More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat
• No
• 563794).