CD141 Mouse anti-Human, Brilliant Violet 711, Clone: 1A4, BD

Description

• The 1A4 monoclonal antibody specifically binds to a 75 kDa, a single chain, type I membrane glycoprotein referred to as thrombomodulin and fetomodulin
• CD141 is expressed on endothelial cells, smooth muscle cells, epithelial cells, synovial lining cells, and keratinocytes
• It is also found on megakaryocytes, dendritic cells, peripheral blood moncytes, and neutrophils, but not on lymphocytes
• Reports indicate that CD141 plays an important role in Protein C activation and the initiation of the Protein C anticoagulant pathway
• Thrombin loses its procagulant function when it binds to CD141, and the CD141/thrombin complex is able to activate Protein C
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.