Granzyme B Mouse anti-Human, Brilliant Violet 421, Clone: GB11, BD

Description

• The GB11 antibody specifically reacts with human granzyme B, a serine protease ofapproximately 32 kDa
• Granzyme B is stored in the granules of cytotoxic T lymphocytes and NK cells along with the pore-forming protein perforin
• In the classic model of target cell lysis, perforins create holes in the target cell membrane allowing entrance of granzymes
• Granzyme B has been shown to act on specific substrates including caspase-3, -7, -9, and -10 which in turn give rise to enzymes that mediate apoptosis
• Granzyme B may also be involved in the hydrolysis of extracellular matrix components
• Detectable levels of granzyme B have been detected in sera from healthy volunteers
• The immunogen used to generate the GB11 hybridoma was human granzyme B isolated from an NK cell line
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.