Bcl-2 Mouse anti-Human, PE-CF594, Clone: Bcl-2/100, BD

Description

• The Bcl-2/100 monoclonal antibody specifically binds to human Bcl-2
• It has been reported not to crossreact with mouse Bcl-2
• A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as the immunogen
• This peptide sequence reportedly is not conserved between human and mouse
• Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenance of tissue homeostasis
• The apoptotic program is characterized by certain morphological features
• These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA
• In the final stages, the dying cells become fragmented into “apoptotic bodies” which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells
• Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals
• Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis in many cell types
• Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis
• Bcl-2 is an approximately 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane
• This antibody is conjugated to BD Horizon™ PE-CF594, which has been developed exclusively by BD Biosciences as a better alternative to PE-Texas Red™
• PE-CF594 excites and emits at similar wavelengths to PE-Texas Red™ yet exhibits improved brightness and spectral characteristics
• Due to PE having maximal absorption peaks at 496nm and 564nm, PE-CF594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as PE-Texas Red™ (eg 610/20-nm filter).