CD314 (NKG2D) Rat anti-Mouse, Brilliant Violet 711, Clone: CX5, BD

Description

• The CX5 monoclonal antibody specifically binds to NKG2D, also known as CD314
• NKG2D is a lectin-like receptor that is detected on resting and IL-2-activated NK cells, activated CD8-positive T lymphocytes, and LPS-activated macrophages, but not on resting T cells or unstimulated macrophages
• NKG2D has little homology to the other members of the NKG2 family, NKG2A, C, and E, and does not form heterodimers with CD94
• On NK cells, NKG2D is an activating receptor that associates with DAP10, an adapter protein that stimulates the PI3 kinase pathway
• An isoform of mouse NKG2D can also associate with the signaling adapter protein DAP12, also known as KARAP ( K iller cell- A ssociated R eceptor- olypeptide), that activates the Syk and ZAP70 tyrosine kinases
• On cytotoxic T cells, NKG2D is a co-stimulatory receptor that associates with DAP10
• The ligands for NKG2D include the minor histocompatibility antigen H60, MULT1 ( M urine UL 16-binding protein- L ike T ranscript ), and the five retinoic acid-inducible proteins Rae-1 a , b , g , d and e
• Interactions of NKG2D with its ligands are involved in the regulation of innate and immune cytotoxic responses to tumor and pathogen-infected cells and in diabetes progression in the NOD mouse
• The CX5 mAb blocks the binding of NKG2D to its ligands
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon™ BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy™5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.