CD196 (CCR6) Mouse anti-Human, Brilliant Violet 786, Clone: 11A9, BD

Description

• The 11A9 monoclonal antibody specifically binds to CCR6
• CCR6 is a seven-transmembrane, G-protein-coupled, glycoprotein receptor that is a member of the beta chemokine receptor family
• CCR6 was clustered as CD196 in the 7th HLDA Workshop
• The human CCR6 gene has been mapped to chromosome 6q27
• CCR6 is a receptor for the CC chemokine CCL20/MIP-3alpha/LARC/Exodus and also binds with lower affinity to and mediates responses to beta-defensin2/hBD-2
• CCR6 is predominantly expressed by B lymphocytes, certain subsets of effector and memory T cells and by immature dendritic cells but not by monocytes, NK cells, or granulocytyes
• Skin-homing CLA (Cutaneous Lymphocyte Antigen)-positive memory T cells, Th1 cells, regulatory T cells and IL-17A-producing Th17 cells predominantly express high levels of CCR6
• CCR6 mediates the trafficking of T, B, and dendritic cells to epithelial sites near the skin and mucosal surfaces during inflammatory and immunological responses
• An N-terminal peptide of human CCR6 was used as an immunogen to generate the 11A9 hybridoma
• The 11A9 antibody does not cross-react with human CCR1, CCR2, CCR3, CCR4, CCR5, CCR7, CCR8, CCR9, CXCR1, CXCR2, CXCR3, CXCR4 and CXCR5 receptors
• This antibody is NOT a neutralizing antibody
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon™ BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).