CD161 Mouse anti-Human, Brilliant Violet 650, Clone: DX12, BD

Description

• The DX12 monoclonal antibody specifically binds to human CD161 which is also known as Natural killer cell surface protein P1A (NKR-P1A)
• CD161 is encoded by the KLRB1 (Killer cell lectin-like receptor subfamily B member 1) gene
• CD161 is a member of the C-type lectin superfamily and is also referred to as C-type lectin domain family 5 member B (CLEC5B)
• CD161 is a 80 kDa disulfide-linked homodimer, type II membrane glycoprotein
• CD161 is expressed mostly on NK cell populations and on subsets of CD4+ and CD8+ αβ T cells, NKT cells and γδ T cells
• Reports indicate that CD161 is expressed preferentially on CD45RO+ T cells, however, it can be found on a subset of thymocytes and fetal liver T cells
• Its function has not been fully elucidated, but reports indicate that NKR-P1A may serve as a specific receptor for some NK cell targets
• The DX12 antibody can reportedly inhibit spontaneous cytotoxicity mediated by certain NK cell clones
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.