CD3 Mouse anti-Rat, Brilliant Violet 605, Clone: 1F4, BD

Description

• The 1F4 antibody reacts with the T-cell receptor-associated CD3 cell-surface antigen found on thymocytes, peripheral T lymphocytes, and dendritic epidermal T cells
• In vitro, immobilized 1F4 mAb induces T-cell proliferation, and soluble mAb synergizes with PMA for T-cell activation
• Pre-incubation of splenocytes with mAb 1F4 blocks staining with mAb G4.18 (Cat
• No
• 559974/554830)
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and Cy™3.5
• Due to the excitation of Cy3.5 by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.