Integrin β7 Rat, Brilliant Violet 650, Clone: FIB504, BD

Description

• FIB504 reacts with mouse integrin β 7 subunit (130 kD) but also cross reacts with human integrin β 7
• Integrin β 7 associates with α 4 (CD49d) expressed on subsets of lymphocytes and thymus
• It also associates with α IEL (CD103) expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes
• Integrin β 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.