TLR4 (CD284) Mouse anti-Human, Brilliant Violet 421, Clone: TF901, BD

Description

• The TF901 monoclonal antibody specifically binds to TLR4 (Toll-like receptor 4) which is also known as CD284
• TLR4 is a 110 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor (TLR) family
• TLR4 is expressed on monocytes, macrophages, granulocytes, dendritic cells and endothelial cells
• TLR4 binds lipopolysaccharide (LPS) which is a major membrane component of Gram-negative bacteria
• It thus acts as an innate immune recognition receptor against many pathogens
• In association with MD-2, LPS binds to and signals through TLR4 receptors by MyD88-dependent and MyD88-independent pathways to induce the cellular production of proinflammatory cytokines
• TF901 antibody binding is not influenced by the human TLR4 D299G/T399I polymorphism
• Preincubation of TLR4-positive cells with the TF901 antibody blocks the subsequent binding of the HTA125 antibody which is also specific for human TLR4
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.