CD4 Mouse anti-Human, BB515, Clone: RPA-T4, BD

Description

• The RPA-T4 monoclonal antibody specifically binds to CD4, a 59 kDa single-chain transmembrane glycoprotein that is expressed on T-helper/inducer cell populations
• CD4 is also expressed on thymocytes subsets and at lower levels on monocytes and macrophages
• CD4 functions as a co-receptor in MHC class II-restricted antigen-induced T cell activation and as a receptor for human immunodeficiency viruses (HIV)
• This antibody binds to the D1 domain (CDR1 and CDR3 epitopes) of the CD4 antigen and reacts with approximately 80% of thymocytes and 45% of peripheral blood lymphocytes
• RPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation
• The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
• With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
• 530/30-nm filter)
• This dye provides a much brighter alternative to FITC with less spillover into the PE detector.