CD272 (BTLA) Mouse anti-Human, Brilliant Violet 421, Clone:  J168-540, BD

Description

• The J168-540 monoclonal antibody specifically binds to human CD272, also known as BTLA (B and T lymphocyte attenuator), an inhibitory receptor expressed in bone marrow and thymus on developing B and T cells
• In the periphery, BTLA is expressed by B cells, T cells, bone marrow-derived dendritic cells, and macrophages
• After T cell activation, BTLA appears to be expressed at higher levels on Th1 cell populations than in Th2 cell populations
• Upon binding the herpesvirus entry mediator (HVEM/LIGHT-R/CD270), CD272 undergoes tyrosine phosphorylation and inhibits T cell proliferation in a BTLA-dependent manner
• CD272 has structural similarities to two other lymphocyte inhibitory receptors, CTLA-4 and PD-1 and is a member of the CD28-like family of coreceptors
• Based on these observations, CD272 is considered to be a negative regulator of lymphocyte activation and/or function
• The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.