IFN-γ Mouse anti-Human, Cynomolgus, Baboon, Rhesus, Brilliant Violet 750, Clone: B27, BD

Description

• The B27 monoclonal antibody specificallybinds to human interferon-γ (IFN-γ), a 14-18 kDa glycoprotein containing 143 amino acid residues
• IFN-γ is a potent multifunctional cytokine produced by several activated cell types including NK, NKT, CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells
• IFN-γ exerts its biological effects through specific binding to the high-affinity IFN-γ receptor complex comprised of IFN-γRα (CD119) and IFN-γRβ subunits
• In addition to its antiviral effects, IFN-γ upregulates a number of lymphoid cell functions including the antimicrobial and anti-tumor responses of macrophages, NK cells, and neutrophils
• In addition, IFN-γ influences the regulation of proliferation, differentiation, and effector responses of B cell and T cell subsets
• These influences can involve IFN-γ's capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves
• B27 is a neutralizing antibody
• The use of B27 antibody for epitope mapping of human IFN-γ has been described
• The B27 antibody has been reported not to bind to denatured IFN-γ
• The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm
• BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• BV750