TNF Mouse anti-Human, Rhesus, Cynomolgus, Baboon, Brilliant Violet 750, Clone: MAb11, BD

Description

• The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF- ) protein
• TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions
• It regulates the growth and differentiation of a variety of cell types
• TNF is cytotoxic for transformed cells when in conjunction with IFN-
• It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts
• The immunogen used to generate the MAb11 hybridoma was recombinant human TNF
• The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF
• The antibody was conjugated to BD Horizon BV750 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 750nm
• BD Horizon Brilliant BV750 can be excited by the violet laser (405 nm) and detected with a 750/30 nm filter with a 740 nm long pass
• Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BV750 reagents
• Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
• BV750