BM28 Mouse, Unlabeled, Clone: 46, BD

Description

• BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band
• The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the “fast” phosphorylated BM28 is the major form found, while in G1 phase, the “slow”, hyperphosphorylated BM28 predominates
• The cellular localization of BM28 is also cell cycle dependent
• In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA
• BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments
• Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication
• When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting