c-Raf Mouse, Unlabeled, Clone: 53, BD

Description

• Raf (c-Raf-1) is a cytoplasmic serine/threonine protein kinase and is a member of a family of proteins which are highly conserved from Drosophila to mammals
• Raf has a critical role in the response to many growth factors including: EGF, PDGF, insulin, IL-2, IL-3, CSF-1, and GM-CSF
• Raf can directly interact with Ras-GTP and subsequently become activated
• Thus Raf plays a prominent role in the Ras signaling pathway by transferring a signal from Ras which has been activated by growth factor receptor-stimulated tyrosine kinase activity
• This leads to the stimulation and activation of a number of other cytoplasmic serine/threonine kinases
• Raf regulates the MAP kinase pathway by phosphorylating and activating MEK, which then phosphorylates and activates MAP kinase (ERK)
• ERK then phosphorylates and activates Rsk
• Raf activity can also be regulated independently of Ras
• Cyclic AMP (cAMP) activation of protein kinase A (PKA) can inhibit growth factor stimulation of Raf.