HIF-1α Mouse anti-Human, Unlabeled, Clone: 54, BD

Description

• Molecular oxygen (O2) is essential for mammalian metabolic processes such as oxidative phosphorylation
• Thus, survival depends upon instantaneous transcriptional modulation of genes that maintain O2 homeostasis
• Transcriptional control of several of these genes is mediated by hypoxia-inducible factor 1 (HIF-1)
• HIF-1 is a heterodimer whose α and β subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors
• Common structural features of these proteins are an N-terminal bHLH DNA-binding domain and multiple PAS domains that confer dimerization ability and target gene specificity
• Members diverge in their C-terminal regions
• HIF-1β is also known as the arylhydrocarbon nuclear translocator which is part of the functional dioxin receptor
• However, HIF-1α functions exclusively to mediate responses to O2 deprivation
• It contains C-terminal and internal transactivation domains
• Although HIF-1 aproteinlevels increase during hypoxia, it is unstable in the presence of O2 due to an oxygen-dependent degradation domain (ODD) that targets it for ubiquitination
• Thus, HIF-1α is essential for functional HIF-1 to mediate gene transcription in response to hypoxia
• Immunofluorescence, Western Blotting