p70 [S6K] Mouse, Unlabeled, Clone: 16, BD

Description

• Increased protein synthesis, an obligatory step in cell proliferation, is regulated by the phosphorylation of a number of translational components, such as ribosomal protein S6
• p70 S6 Kinase (p70 [S6K]), a cytosolic kinase that is primarily responsible for S6 phosphorylation, belongs to a family of at least six S6 kinases
• p70 [S6K] and p85 [S6K] are two isoforms of the same kinase
• Although they are encoded by the same gene, p80 [S6K] contains a 23 amino acid N-terminal extension that serves as a nuclear localization sequence
• These kinases are located in a p21 ras-independent signaling pathway and are regulated by Ser/Thr phosphorylation in response to mitogenic stimulation
• Activation of p70 [S6K] requires sequential phosphorylations within the autoinhibitory domain and at Thr 389
• p70 [S6K] associates with PDK-1 and PKC? to form a possible PI3-K signaling complex
• The immunosuppressive agent rapamycin forms an intracellular complex with FKBP12 and FRAP (RAFT1) that inhibits phosphorylation of p70 [S6K] at Thr 389 and, in turn, prevents enzyme activation
• Thus, p70 [S6K] is an important regulator of cell proliferation and a potential target of agents that modify the immune and proliferative responses
• p70 [S6K] has been reported to be observable migrating within a range of 60-70 kD by western blotting
• Host Species: Mouse Clone: 16 Isotype: IgG1 Species Reactivity: Rat Immunogen: Rat p70 [S6K] aa
• 2-121 Formula Weight [Chemical]: 60-70kDa Immunofluorescence, Western Blotting