DARPP-32 Mouse, Unlabeled, Clone: 15, BD

Description

• Dopaminergic signaling in midbrain neurons is essential to multiple brain functions and involves the activation of dopamine receptors, such as D1 and D2, which regulate the phosphorylation state of DARPP-32 (dopamine and cyclic AMP-regulated phospho-protein of Mr = 32,000)
• D1 receptor ligation causes activation of PKA and phosphorylation of DARPP-32 at Thr-34, which converts DARPP-32 to a potent inhibitor of protein phosphatase 1 (PP1)
• In addition, DARPP-32 is converted to an inhibitor of PKA via phosphorylation at Thr-75 by cyclin-dependent kinase 5 (Cdk5)
• D2 receptor ligation inhibits PKA and activates protein phosphatase 2B/calcineurin causing dephosphorylation of DARPP-32
• The major function of DARPP-32 may be to inhibit the activity of PP1, which controls the phosphorylation state of neurotransmitter receptors, ion channels, ion pumps, and transcription factors
• DARPP-32 -/- mice are defective in the physiologicial and behavioral responses to dopamine
• Thus, DARPP-32, a bifunctional signal transduction molecule that differentially controls a Ser/Thr kinase and a Ser/Thr phosphatase, is a critical element of dopaminergic neurotransmission and normal brain function
• Host Species: Mouse Clone: 15 Isotype: IgG1 Species Reactivity: Rat Immunogen: Human DARPP-32 aa
• 70-181 Formula Weight [Chemical]: 32kDa Immunofluorescence, Western Blotting