CD86 Mouse anti-Baboon, Human, Rhesus, , Brilliant Ultraviolet 737, Clone: 2331 (FUN-1), BD Horizon™

Description

• The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells
• Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (anti-CD86, Cat
• No
• 555663) antibodies specifically recognize the same molecule, they react with different epitopes
• CD86 is the second ligand for CD28 and CTLA-4 and may play an important role in co-stimulation of T cells in primary immune response
• The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies
• The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).