CD26 Rat anti-Mouse, Brilliant Violet 421, Clone: H194-112, BD Optibuild™

Description

• The H194-112 monoclonal antibody specifically binds to CD26, which is also known as, Thymocyte-activating molecule (THAM), or dipeptidyl peptidase IV (DPP IV, Dpp4)
• CD26 is a ∽220- kDa dimer formed of identical type-II transmembrane core polypeptides which undergo variable post-translational modifications
• It is a multi-functional molecule with both ectopeptidase and signal-transducing activities
• Studies with specific DPP IV inhibitors suggest that the enzymatic activity is involved in the mediation of T-cell activation events
• The expression of CD26 is developmentally regulated in the thymus
• Resting lymphoid cells of the bone marrow and peripheral B and T lymphocytes express low levels of CD26; bone-marrow and peritoneal myeloid cells do not
• CD26 is also found on epithelial cells in the kidney, liver, small intestine, and lung
• Cross-linked H194-112 mAb induces proliferation of immature and mature thymocytes in the presence of either IL-1 plus IL-2 or PMA; addition of IL-2 or IL-4 to PMA further enhances the activation
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.