CD274 Mouse anti-Human, BUV395, Clone: MIH1, BD Optibuild™

Description

• PD-L1 and PD-L2 are newly discovered members of the B7 family are the ligands for the program death 1 (PD-1) receptor
• They are expressed on immature dendritic cells and mature dendritic cells
• PD-L1, also called B7-H1, is expressed on antigen-presenting cells, including IFN- γ-stimulated monocytes, and activated human and murine dendritic cells
• Monoclonal antibodies that block PD-L1 and PD-L2 on dendritic cells result in enhanced T cell proliferation and cytokine production
• PD-L1 is also expressed on placental trophoblasts, myocardial endothelium, cortical thymic epithelial cells, and on most carcinomas
• Studies show overlapping functions of PD-L1 and PD-L2 and indicate an important role for the PD-L:PD-1 pathway in regulating T cell responses
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.