CD274 Mouse anti-Human, Brilliant Violet 605, Clone: MIH1, BD Optibuild™

Description

• PD-L1 and PD-L2 are newly discovered members of the B7 family are the ligands for the program death 1 (PD-1) receptor
• They are expressed on immature dendritic cells and mature dendritic cells
• PD-L1, also called B7-H1, is expressed on antigen-presenting cells, including IFN- γ-stimulated monocytes, and activated human and murine dendritic cells
• Monoclonal antibodies that block PD-L1 and PD-L2 on dendritic cells result in enhanced T cell proliferation and cytokine production
• PD-L1 is also expressed on placental trophoblasts, myocardial endothelium, cortical thymic epithelial cells, and on most carcinomas
• Studies show overlapping functions of PD-L1 and PD-L2 and indicate an important role for the PD-L:PD-1 pathway in regulating T cell responses
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.