CD22 Mouse anti-Human, Brilliant Violet 605, Clone: HIB22, BD Optibuild™

Description

• The HIB22 monoclonal antibody specifically binds to CD22
• CD22 is a 130-140 kDa glycosylated type I integral membrane protein present on the surface of mature B cells
• CD22 is expressed in the cytoplasm of virtually all B cells except plasma cells
• CD45RO antigen on T cells and CD75 antigen on B cells have been identified as ligands for CD22
• CD22 has been reported to participate in B-cell activation and also as an adhesion molecule
• Although the immunobiology of this antigen has not been fully elucidated, reports indicate that ligation of CD22 induces constitutive internalization of the molecule followed by complete degradation
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.