CD32 Mouse anti-Human, Brilliant Violet 605, Clone: FLI8.26 (2003), BD Optibuild™

Description

• The FLI8.26 monoclonal antibody specifically recognizes CD32 which is also known as Fcγreceptor type II (FcγRII)
• CD32 is a type I transmembrane glycoprotein that serves as a low affinity receptor for aggregated IgG
• The FLI8.26 antibody recognizes a, b, and c forms of CD32 that are encoded by separate genes: CD32a/ FcγRIIa ( FCGR2A ), CD32b/ FcγRIIb ( FCGR2B )
• These forms are differentially expressed on monocytes, granulocytes, T cells, B cells, NK cells, or platelets
• These receptors play various roles in mediating and regulating inflammation and immunity including phagocytosis, cytotoxicity, degranulation, or platelet activation
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.