CD32 Mouse anti-Human, Brilliant Violet 650, Clone: FLI8.26 (2003), BD Optibuild™

Description

• The FLI8.26 monoclonal antibody specifically recognizes CD32 which is also known as Fcγreceptor type II (FcγRII)
• CD32 is a type I transmembrane glycoprotein that serves as a low affinity receptor for aggregated IgG
• The FLI8.26 antibody recognizes a, b, and c forms of CD32 that are encoded by separate genes: CD32a/ FcγRIIa ( FCGR2A ), CD32b/ FcγRIIb ( FCGR2B )
• These forms are differentially expressed on monocytes, granulocytes, T cells, B cells, NK cells, or platelets
• These receptors play various roles in mediating and regulating inflammation and immunity including phagocytosis, cytotoxicity, degranulation, or platelet activation
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.