CD19 Mouse anti-Human, Brilliant Violet 605, Clone: HIB19, BD Optibuild™

Description

• The HIB19 monoclonal antibody specifically binds to the 95 kDa type I transmembrane CD19 glycoprotein
• CD19 is expressed during all stages of B-cell maturation and differentiation, except on plasma cells
• CD19 is also present on follicular dendritic cells
• It is not found on T cells or on normal granulocytes
• CD19 is a signal transduction molecule that regulates B cell development, activation, proliferation and differentiation
• It associates with the complement receptor 2 (CD21), TAPA-1 (CD81), Leu 13, and/or MHC class II to form a signal transduction complex on the surface of B cells
• Anti-CD19 clone HIB19 partially blocks the binding of clone B43, another CD19-specific monoclonal antibody
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.