CD19 Mouse anti-Human, Brilliant Violet 711, Clone: HIB19, BD Optibuild™

Description

• The HIB19 monoclonal antibody specifically binds to the 95 kDa type I transmembrane CD19 glycoprotein
• CD19 is expressed during all stages of B-cell maturation and differentiation, except on plasma cells
• CD19 is also present on follicular dendritic cells
• It is not found on T cells or on normal granulocytes
• CD19 is a signal transduction molecule that regulates B cell development, activation, proliferation and differentiation
• It associates with the complement receptor 2 (CD21), TAPA-1 (CD81), Leu 13, and/or MHC class II to form a signal transduction complex on the surface of B cells
• Anti-CD19 clone HIB19 partially blocks the binding of clone B43, another CD19-specific monoclonal antibody
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.