CD44 Mouse anti-Human, Brilliant Violet 711, Clone: G44-26 (also known as C26), BD Optibuild™

Description

• The G44-26 monoclonal antibody specifically binds to the 80-95 kDa, glycosylated type I transmembrane protein, also known as phagocytic glycoprotein-1 (Pgp-1)
• CD44 is the receptor for hyaluronic acid
• CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets
• CD44 is also called extracellular matrix receptor type III and has functional roles in cell migration, lymphocyte homing and adhesion during hematopoiesis and lymphocyte activation
• This antibody is suitable for staining formalin-fixed, paraffin-embedded tissue sections using citrate buffer pretreatment
• This antibody recognizes the epitope 1 of CD44 antigen according to the HLDA workshop studies
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.