CD21 Mouse anti-Human, Brilliant Violet 605, Clone: B-ly4, BD Optibuild™

Description

• The B-ly4 monoclonal antibody specifically binds to CD21, a 145 kDa glycosylated type I integral membrane protein
• CD21 is a receptor for the C3d complement fragment and for Epstein-Barr virus (EBV)
• CD21 is expressed on mature B cells, follicular dendritic cells, and some epithelial cells
• It is also weakly expressed on the subset of mature T cells and thymocytes
• CD21 plays a role in B-cell activation and proliferation
• It may also play a role in modulating the function of T cells in the immune response to infections by lymphotropic viruses
• Recently, CD21 was found to be part of a large complex containing CD19, CD81, and possibly other molecules
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.