CD21 Mouse anti-Human, Brilliant Violet 650, Clone: B-ly4, BD Optibuild™

Description

• The B-ly4 monoclonal antibody specifically binds to CD21, a 145 kDa glycosylated type I integral membrane protein
• CD21 is a receptor for the C3d complement fragment and for Epstein-Barr virus (EBV)
• CD21 is expressed on mature B cells, follicular dendritic cells, and some epithelial cells
• It is also weakly expressed on the subset of mature T cells and thymocytes
• CD21 plays a role in B-cell activation and proliferation
• It may also play a role in modulating the function of T cells in the immune response to infections by lymphotropic viruses
• Recently, CD21 was found to be part of a large complex containing CD19, CD81, and possibly other molecules
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.