IgG Mouse anti-Human, Brilliant Violet 650, Clone: G18-145, BD Optibuild™

Description

• IgG is an important component of the humoral immune response, helping to control infection
• IgG is created by plasma B-cells and may be found in extracellular fluids, such as blood, lymph, peritoneal, and cerebrospinal fluids
• IgG monomers consist of two light and two heavy chains containing two antigen binding sites
• There are four IgG subclasses found in human, mouse and rat species, which include IgG1, IgG2, IgG3 and IgG4
• The G18-145 monoclonal antibody specifically binds to the heavy chain of human immunoglobulin G subclasses: IgG1, IgG2, IgG3 and IgG4
• The G18-145 antibody has been reported not to react with the heavy chains of other human immunoglobulin isotypes
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.