CD30 Hamster anti-Mouse, Brilliant Violet 711, Clone: mCD30.1 (also known as 2SH12-5F-2D), BD Optibuild™

Description

• The mCD30.1 monoclonal antibody specifically recognizes CD30
• CD30 is also known as Tumor necrosis factor receptor superfamily, member 8 (Tnfrsf8)
• The CD30 molecule is predominantly expressed by activated T lymphocytes, with its expression peaking at day 4-5 on spleen cells activated with plate-bound anti-CD3e antibody
• The mCD30.1 antibody reacts with a majority of CD8+ T cells, as well as some CD4+ T cells in these cultures
• Expression of CD30 on activated T lymphocytes is regulated by CD28 and cytokines
• Its TNF-superfamily ligand, CD30L or CD153, is also expressed on activated T lymphocytes
• By northern blot analysis, mouse Cd30 mRNA is detected in the thymus and in 72-hour pokeweed mitogen- and Con A-activated spleen cells, but not in the lung, brain, kidney, liver, bone marrow, unactivated spleen, or 72-hour LPS-activated splenocytes.1 It has also been reported that CD30 is expressed on naive B lymphocytes, it is not detectable after activation, and it starts to return after 72 hours following activation
• Reports suggest that signaling through the CD30 molecule may be important in cytokine production by CD8+ CTL lines and may play a role in the regulation of Th1 and Th2 cytokine secretion by CD4+ and CD8+ T cells
• It has also been proposed that CD30 plays an important role in the negative selection of thymocytes and protects against autoimmunity
• Members of the TNFR family and their ligands are involved in the induction of diverse biological responses in lymphocytes, including differentiation, proliferation, and cellular death
• In humans, CD30 was initially identified in Hodgkin and Reed-Sternberg cells in Hodgkin’s disease patients and subsequently was found on neoplastic cells of certain types of non-Hodgkin’s lymphomas
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.