MSR1 (CD204) Mouse anti-Human, Brilliant Violet 605, Clone: U23-56, BD Optibuild™

Description

• The U23-56 monoclonal antibody specifically binds to Macrophage scavenger receptor 1 (MSR1) which is also known as CD204, Scavenger receptor A (SRA), Scavenger receptor class A member 1 (SCARA1), or Macrophage acetylated LDL receptor I and II
• MSR1 is a trimeric type II transmembrane glycoprotein that belongs to the type 1 class A scavenger receptor family
• MSR1 functions as a scavenger receptor that enables mononuclear phagocytes to take up and respond to a variety of negatively charged macromolecules including oxidized and acetylated low density lipoproteins
• MSR1 also functions in innate immunity as a pattern recognition receptor
• MSR1 allows macrophages to recognize and respond to microbial products, such as lipopolysaccharide and polyribonucleic acids, by the production of cytokines and inflammatory mediators
• MSR1 also reportedly serves as a coreceptor that regulates the signaling of some Toll-like receptors including TLR-3, TLR-4, and TLR-9
• MSR1 has been associated with several diseases including atherosclerosis, Alzheimer’s disease, ischemic injury, and sepsis
• The U23-56 antibody was tested by flow cytometric and Western blot analyses of MSR1-transfected and untransfected cells to verify its specificity
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.