MSR1 (CD204) Mouse anti-Human, Brilliant Violet 711, Clone: U23-56, BD Optibuild™

Description

• The U23-56 monoclonal antibody specifically binds to Macrophage scavenger receptor 1 (MSR1) which is also known as CD204, Scavenger receptor A (SRA), Scavenger receptor class A member 1 (SCARA1), or Macrophage acetylated LDL receptor I and II
• MSR1 is a trimeric type II transmembrane glycoprotein that belongs to the type 1 class A scavenger receptor family
• MSR1 functions as a scavenger receptor that enables mononuclear phagocytes to take up and respond to a variety of negatively charged macromolecules including oxidized and acetylated low density lipoproteins
• MSR1 also functions in innate immunity as a pattern recognition receptor
• MSR1 allows macrophages to recognize and respond to microbial products, such as lipopolysaccharide and polyribonucleic acids, by the production of cytokines and inflammatory mediators
• MSR1 also reportedly serves as a coreceptor that regulates the signaling of some Toll-like receptors including TLR-3, TLR-4, and TLR-9
• MSR1 has been associated with several diseases including atherosclerosis, Alzheimer’s disease, ischemic injury, and sepsis
• The U23-56 antibody was tested by flow cytometric and Western blot analyses of MSR1-transfected and untransfected cells to verify its specificity
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.