H-2Dd Mouse anti-Mouse, Brilliant Violet 605, Clone: 34-2-12, BD Optibuild™

Description

• The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]
• The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin
• It cross-reacts with cells of the C3H.LG/Ckc strain
• Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed
• Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]
• However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d]
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.