H-2Dd Mouse anti-Mouse, Brilliant Violet 711, Clone: 34-2-12, BD Optibuild™

Description

• The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]
• The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin
• It cross-reacts with cells of the C3H.LG/Ckc strain
• Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed
• Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]
• However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d]
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.