CD156c Mouse anti-Human, Brilliant Violet 421, Clone: 11G2, BD Optibuild™

Description

• The 11G2 monoclonal antibody specifically binds to CD156c which is also known as Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10)
• CD156c is a ñ70 kDa type I transmembrane glycoprotein that belongs to the ADAM family
• It is widely expressed on hematopoietic and non-hematopoietic cells
• CD156c is expressed on the plasma membrane, as well as by membranes within intracellular compartments and exosomes
• CD156c serves as a broadly-reactive endopeptidase that cleaves membrane-bound proteins in a process known as ectodomain shedding or release
• It can cleave transmembrane molecules into soluble forms and thereby regulate the functions of a variety of different receptors and ligands that are involved in cellular signaling and adhesion
• These include membrane TNF, CX3CL1, CXCL16, Ephrin-A2, Notch and Delta molecules, cadherins, CD23, CD44, IL-6 Receptor/CD126, CD171, and amyloid precursor protein
• CD156c expression may be constitutive or induced, such as in inflamed tissues, including arthritic joints and in the nervous system
• Dysregulated CD156c expression may be associated with certain cancers and Alzheimer’s disease
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.