CD156c Mouse anti-Human, Brilliant Violet 650, Clone: 11G2, BD Optibuild™

Description

• The 11G2 monoclonal antibody specifically binds to CD156c which is also known as Disintegrin and metalloproteinase domain-containing protein 10 (ADAM10)
• CD156c is a ñ70 kDa type I transmembrane glycoprotein that belongs to the ADAM family
• It is widely expressed on hematopoietic and non-hematopoietic cells
• CD156c is expressed on the plasma membrane, as well as by membranes within intracellular compartments and exosomes
• CD156c serves as a broadly-reactive endopeptidase that cleaves membrane-bound proteins in a process known as ectodomain shedding or release
• It can cleave transmembrane molecules into soluble forms and thereby regulate the functions of a variety of different receptors and ligands that are involved in cellular signaling and adhesion
• These include membrane TNF, CX3CL1, CXCL16, Ephrin-A2, Notch and Delta molecules, cadherins, CD23, CD44, IL-6 Receptor/CD126, CD171, and amyloid precursor protein
• CD156c expression may be constitutive or induced, such as in inflamed tissues, including arthritic joints and in the nervous system
• Dysregulated CD156c expression may be associated with certain cancers and Alzheimer’s disease
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.