MAdCAM-1 Rat anti-Mouse, Brilliant Violet 786, Clone: MECA-367, BD Optibuild™

Description

• The MECA-367 monoclonal antibody specifically recognizes mucosal vascular addressin MAdCAM-1
• In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes
• In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria; it is also expressed on sinus-lining cells in the spleen
• MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis
• Furthermore, there is evidence that IFN-γcan induce MAdCAM-1 expression in non-mucosal sites in adult mice
• MAdCAM-1 is a predominant ligand for integrinα4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin)
• MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro /and lymphocyte homing to Peyer's patch HEV in vivo /
• Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter)
• BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm
• BV786 offers a bright choice for the 6th detector off the violet laser.