MAdCAM-1 Rat anti-Mouse, Brilliant Violet 421, Clone: MECA-367, BD Optibuild™

Description

• The MECA-367 monoclonal antibody specifically recognizes mucosal vascular addressin MAdCAM-1
• In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes
• In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria; it is also expressed on sinus-lining cells in the spleen
• MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice4 and is involved in the development of diabetes and insulitis
• Furthermore, there is evidence that IFN-γcan induce MAdCAM-1 expression in non-mucosal sites in adult mice
• MAdCAM-1 is a predominant ligand for integrinα4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin)
• MECA-367 mAb binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro /and lymphocyte homing to Peyer's patch HEV in vivo /
• Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.